identification of candida species isolated from vulvovaginal candidiasis using pcr-rflp

Authors

reyhaneh roshan

department of microbiology, karaj branch, islamic azad university, karaj, iran.سازمان اصلی تایید شده: دانشگاه آزاد اسلامی کرج (islamic azad university of karaj) azar sabokbar

department of microbiology, karaj branch, islamic azad university, karaj, iran.سازمان اصلی تایید شده: دانشگاه آزاد اسلامی کرج (islamic azad university of karaj) hamid badali

department of medical parasitology and mycology/molecular and cell biology research center; school of medicine, mazandaran university of medical sciences, sari, iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (mazandaran university of medical sciences)

abstract

vulvovaginal candidiasis (vvc) is a common disease among women worldwide, therefore, accurate and rapid diagnosis of causative agents based on molecular techniques utilizing amplification of target dna is highly recomendad for epidemiological purposes and for effective treatment. the aim of this study was to identify clinically candida species from vvc patients by restriction fragment length polymorphism (pcr-rflp). a total of 155 patients with suspicious symptoms of vvc were screened. candida strainsisolated from specimens initially were identified by phenotypic methods and confirmed by molecular approaches based on pcr-rflp. fifty one (34%) strains of candida were obtained from specimens collected from vvc patients. c. albicans was the most frequently isolated species (86.2%) followed by non-albicans, including c. glabrata (7.9%), c. kefir (3.9%), and c. tropicalis (1.9%). the restriction patterns of each candida species were perfectly specific. the identification of candida species in vvc due to developing antifungal resistance is very significant for appropriate treatment and to prevent the spread of vvc.

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Journal title:
international journal of molecular and clinical microbiology

جلد ۴، شماره ۲، صفحات ۴۰۶-۴۱۰

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